CASP6 mouse mab MP31730 [M3B7E10]

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family of enzymes. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic acid residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms.

Application

Protein:Caspase-6

Validation

1-WESTERN BLOT

WESTERN BLOT ANALYSIS USING CASP6 MAB AGAINST HUMAN CASP6 (AA: 194–293) RECOMBINANT PROTEIN. (EXPECTED MW IS 37.2 KDA)

2-WESTERN BLOT

WESTERN BLOT ANALYSIS USING CASP6 MAB AGAINST HEK293 (1) AND CASP6 (AA: 194–293)-HIGGFC TRANSFECTED HEK293 (2) CELL LYSATE.

3-FLOW CYTOMETRIC ANALYSIS

FLOW CYTOMETRIC ANALYSIS OF HELA CELLS USING CASP6 MOUSE MAB (GREEN) AND NEGATIVE CONTROL (RED).

4-Elisa

BLACK LINE: CONTROL ANTIGEN (100 NG);PURPLE LINE: ANTIGEN (10NG); BLUE LINE: ANTIGEN (50 NG); RED LINE:ANTIGEN (100 NG)

	数据来源	J Hazard Mater (2020 Apr). Figure 6. Cell Counting Kit-8 (AbMole BioScience, Houston, TX, USA)
	方法	Cell viability assay
	细胞系	LO2 and HEK 293 cells
	浓度	-
	处理时间	-
	实验结果	As shown in Fig. 6, PAT-J significantly reduced the viability of the two cells compared with the JUICE group, while the cell viability of the PAT-JP and LS-J2 treated groups did not change significantly.
	评级