Anti-BTK Antibody (phospho-Ser180) MX-C4243
Details
Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members.
| immunogen | BTK antibody was raised against synthetic phosphopeptide (KLH coupled) corresponding to residues around Ser180 of human Btk. Species Sequence Homology: Mouse - 100%. |
|---|---|
| conjugations | Unconjugated |
| epitope | pSer180 |
| furification | Immunoaffinity purified |
| host | Mouse |
| antibody | Anti-BTK Antibody (phospho-Ser180) MX-C4243 |
| modifications | Unmodified |
| reactivity | Human |
| ncbi | Q06187 |
| reconstitution | Mouse (at least 90% immunogen sequence identity) |
| synonyms | NM_000061 NP_000052.1 |
| restrictions | For research use only. |
| specificity | Recognizes overexpressed levels of human Btk only when phosphorylated at Ser180 (Mr 77kD). This antibody cross-reacts with some other proteins. |
| storage | Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles. |
| type | Primary |
| usage | Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:1000. For Western Blot, USBio recommends using immunoprecipitated Btk. Immunoprecipitation: 1:25. |
| target | BTK |
| predicted | pSer180 |
| format | Unconjugated |
| clonality | IgG Monoclonal |
| application | Western blot, Immunoprecipitation, ELISA |