Serum
FBS basic information
Important terms to know when evaluating FBS
| Terms | Definitions |
|---|---|
| 9CFR virus testing | Virus panel testing according Code of Federal Regulations, (CFR), Title 9, Part 113.53 (c) [113.46, 113.47]. Detected by fluorescent antibody. |
| Biochemical and hormone profile | Quantification of biochemical and hormonal (estradiol, insulin, progesterone, testosterone, and thyroxine) profiling that may have impact on cell culture. |
| BSE status | BSE (bovine spongiform encephalopathy) is a disease for which the OIE (The World Organisation for Animal Health) has established official recognition of the sanitary status of countries and zones. Regions that have negligible risk of bovine spongiform encephalopathy (mad cow disease) have lesser biosafety risk for import. |
| EMA virus testing | Virus panel testing according Code of Federal Regulations, (CFR), Title 9, Part 113.53 (c) [113.46, 113.47]. Detected by fluorescent antibody. |
| Endotoxin testing | Endotoxin is directly related to the quality of collection and processing of serum, the higher the level, the more introduction to gram-negative bacteria. |
| Filtration | Triple (0.1 µm) filtration: Aseptic process that has been validated to ensure that all products meet the industry standard sterility assurance level of 10-3. |
| Haemoglobin/hemoglobin | Indicator of proper and/or improper collection and processing of blood and/or serum. |
| Mycoplasma and Mycoplasma supplemental testing (H-Stain) | Direct culture and Hoechst stain. Testing indicates Mycoplasma - Not Detected. |
| Origin confirmation | We use a proprietary sera fingerprinting technology for Gibco sera to confirm FBS origin and eliminate the potential for counterfeit product. Learn more |
| Osmolality | Osmolality of FBS, a measure of the concentration of solutes like salts and sugars, should be similar to culture media to avoid osmotic shock that may impact cell viability. |
| Performance: relative growth promotion (RGP) | The growth promotion assay measures the ability of each FBS lot to support proliferation of fastidious human diploid fibroblasts through multiple subcultures. |
| pH: relative pH promotion | Serum acts as a buffer in the cell culture system; the pH is tested to ensure accurate cell culture quality and performance. |
| Total protein | FBS is rich in a variety of proteins that can impact cultured cells; the total protein in serum is measured by taking a chemistry panel of the serum. |
| Traceability | Complete traceability back to original source. ISIA Traceability Certified. |
Standard bovine definitions
| Terms | Definitions |
|---|---|
| Serum | Serum is the liquid fraction of clotted blood. It is depleted of cells, fibrin and clotting factors. Serum differs from plasma in that anti-coagulant is never added to the blood after collection from the animal. Serum is prepared by centrifuging until the clot and remaining blood cells are separated from the liquid phase. The serum is then removed and stored frozen pending further processing. |
| Fetal Bovine Serum | Semi-processed Fetal Bovine Serum Fetal bovine serum (FBS) is obtained as described above from the blood of fetuses of healthy, pre-partum bovine dams that have been ante- and/or post-mortem veterinary inspection. It is collected in abattoirs inspected by the competent authority in the country of origin. Fetal blood is collected aseptically using cardiac puncture, thereby reducing the risk of microbial contamination and resultant endotoxins. Collection occurs in an area of the abattoir specifically set aside for this purpose to minimize the risk of contamination by other fluids. |
| Specialty Fetal Bovine Serum | This is semi-processed FBS or sterile filtered FBS that has been subjected to one or more modification processes. Examples are Dialyzed, Charcoal Stripped, Ultra-low IgG, ES Cell, MSC and Exosome-Depleted. |
| Newborn Calf Serum | Newborn Calf Serum (NBCS) is defined as the liquid fraction of clotted blood derived from healthy, slaughtered bovine calves aged less than 20 days, deemed fit for human consumption through ante- and/or post- mortem inspection. It is collected in abattoirs inspected by the competent authority of the country of origin. There are no deletions or additions (including preservatives) allowed. |
| Donor Bovine Serum (also known Donor Calf Serum) | Donor-sourced Bovine Serum (DBS) is defined as the liquid fraction of clotted blood derived from healthy cattle 12 months of age or older from controlled donor herds whose health status is confirmed by regular inspection by competent, legally authorized veterinarians. |
| Bovine Serum (also known as Adult Bovine or Calf Serum) | Bovine Serum is defined as the liquid fraction of clotted blood derived from healthy, slaughtered cattle 12 months of age or older, deemed to be fit for human consumption by ante- and/or post- mortem inspection. It is collected in abattoirs inspected by the competent authority of the country of origin. |
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