CASP6 mouse mab MP31730 [M3B7E10]

Details

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family of enzymes. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic acid residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms.

Application

Application	FCM, WB, ELISA, ICC
ELISA	1/10000
ICC (Immunocytochemistry)	1/100- 1/500
IHC_P (Immunohistochemistry)	N/A
FCM (Flow Cytometry)	1/200 - 1/400
WB (Cell Lysate)	1/200 - 1/1000
WB (Recombinant Protein)	1/500 - 1/2000

Protein:Caspase-6

Function	Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.
Catalytic activity	Strict requirement for Asp at position P1 and has a preferred cleavage sequence of Val-Glu-His-Asp-\|-. EC:3.4.22.59
Activity regulation	Activation is suppressed by phosphorylation at Ser-257.
Protein names	Recommended name: Caspase-6 (EC:3.4.22.59) Short name: CASP-6 Alternative name(s): Apoptotic protease Mch-2 Cleaved into the following 2 chains: Caspase-6 subunit p18 Caspase-6 subunit p11
Subcellular location	Cytoplasm
Mutagenesis	S → A: Suppression of caspase-6 activation. Position(s):257
Post-translational modification	Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.
Interaction	Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 11 kDa (p11) subunit. Interacts with BIRC6/bruce.
Family	Belongs to the peptidase C14A family.

Validation

1-WESTERN BLOT

WESTERN BLOT ANALYSIS USING CASP6 MAB AGAINST HUMAN CASP6 (AA: 194–293) RECOMBINANT PROTEIN. (EXPECTED MW IS 37.2 KDA)

2-WESTERN BLOT

WESTERN BLOT ANALYSIS USING CASP6 MAB AGAINST HEK293 (1) AND CASP6 (AA: 194–293)-HIGGFC TRANSFECTED HEK293 (2) CELL LYSATE.

3-FLOW CYTOMETRIC ANALYSIS

FLOW CYTOMETRIC ANALYSIS OF HELA CELLS USING CASP6 MOUSE MAB (GREEN) AND NEGATIVE CONTROL (RED).

4-Elisa

BLACK LINE: CONTROL ANTIGEN (100 NG);PURPLE LINE: ANTIGEN (10NG); BLUE LINE: ANTIGEN (50 NG); RED LINE:ANTIGEN (100 NG)

	数据来源	J Hazard Mater (2020 Apr). Figure 6. Cell Counting Kit-8 (AbMole BioScience, Houston, TX, USA)
	方法	Cell viability assay
	细胞系	LO2 and HEK 293 cells
	浓度	-
	处理时间	-
	实验结果	As shown in Fig. 6, PAT-J significantly reduced the viability of the two cells compared with the JUICE group, while the cell viability of the PAT-JP and LS-J2 treated groups did not change significantly.
	评级

immunogen	Purified recombinant fragment of human CASP6 (AA: 194–293) expressed in E. Coli.
conjugations	No
epitope	human CASP6 (AA: 194–293)
host	mouse
reactivity	human
reconstitution	Purified antibody in PBS with 0.05% sodium azide
storage	-20

Product Tags

Use spaces to separate tags. Use single quotes (') for phrases.