CASP6 mouse mab MP31731
Details
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family of enzymes. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic acid residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms.
Application
| Application | FCM, WB, ELISA, ICC |
|---|---|
| ELISA | 1/10000 |
| ICC (Immunocytochemistry) | 1/100- 1/500 |
| IHC_P (Immunohistochemistry) | N/A |
| FCM (Flow Cytometry) | 1/200 - 1/400 |
| WB (Cell Lysate) | 1/200 - 1/1000 |
| WB (Recombinant Protein) | 1/500 - 1/2000 |
Protein:Caspase-6
| Function | Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death. |
|---|---|
| Catalytic activity | Strict requirement for Asp at position P1 and has a preferred cleavage sequence of Val-Glu-His-Asp-|-. EC:3.4.22.59 |
| Activity regulation | Activation is suppressed by phosphorylation at Ser-257. |
| Protein names | Recommended name: Caspase-6 (EC:3.4.22.59) Short name: CASP-6 Alternative name(s): Apoptotic protease Mch-2 Cleaved into the following 2 chains: Caspase-6 subunit p18 Caspase-6 subunit p11 |
| Subcellular location | Cytoplasm |
| Mutagenesis | S → A: Suppression of caspase-6 activation. Position(s):257 |
| Post-translational modification | Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits. |
| Interaction | Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 11 kDa (p11) subunit. Interacts with BIRC6/bruce. |
| Family | Belongs to the peptidase C14A family. |
Validation
1-WESTERN BLOT
WESTERN BLOT ANALYSIS USING CASP6 MAB AGAINST HUMAN CASP6 (AA: 194–293) RECOMBINANT PROTEIN. (EXPECTED MW IS 37.2 KDA)
2-WESTERN BLOT
WESTERN BLOT ANALYSIS USING CASP6 MAB AGAINST HEK293 (1) AND CASP6 (AA: 194–293)-HIGGFC TRANSFECTED HEK293 (2) CELL LYSATE.
3-FLOW CYTOMETRIC ANALYSIS
FLOW CYTOMETRIC ANALYSIS OF HELA CELLS USING CASP6 MOUSE MAB (GREEN) AND NEGATIVE CONTROL (RED).
4-Elisa
BLACK LINE: CONTROL ANTIGEN (100 NG);PURPLE LINE: ANTIGEN (10NG); BLUE LINE: ANTIGEN (50 NG); RED LINE:ANTIGEN (100 NG)
| immunogen | Purified recombinant fragment of human CASP6 (AA: 194–293) expressed in E. Coli. |
|---|---|
| conjugations | No |
| epitope | human CASP6 (AA: 194–293) |
| host | mouse |
| reactivity | human |
| reconstitution | Purified antibody in PBS with 0.05% sodium azide |
| storage | -20 |